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pBGSA C5aR1-EGFP
(Plasmid #62612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62612 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 62612-D50 50 μg of DNA in Tris buffer $495
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DNA 62612-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBGSA
  • Backbone manufacturer
    Bruce Granger, PMID 8867788
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 9140
  • Modifications to backbone
    Modified from pBS SK+
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C5aR1
  • Alt name
    C5aR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1940
  • GenBank ID
    M62505
  • Entrez Gene
    C5AR1 (a.k.a. C5A, C5AR, C5R1, CD88)
  • Promoter SRalpha
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer cttctaggcctgtacgg
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGSA C5aR1-EGFP was a gift from Heini Miettinen (Addgene plasmid # 62612 ; http://n2t.net/addgene:62612 ; RRID:Addgene_62612)

  • For your References section:

    Different endocytosis pathways of the C5a receptor and the N-formyl peptide receptor. Suvorova ES, Gripentrog JM, Miettinen HM. Traffic. 2005 Feb;6(2):100-15. 10.1111/j.1600-0854.2004.00256.x PubMed 15634211
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