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Addgene

pCas9-CR4 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 62655 with XhoI and HindIII-HF.

We expected the following banding patterns:
XhoI single digest: ~6.8kb
XhoI / HindIII-HF double digest: ~ 3.6, 2.3, and 0.8kb

We see the expected result, although the lowest band is quite faint.