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(Plasmid #62805)


Item Catalog # Description Quantity Price (USD)
Plasmid 62805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 7066
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    E2F transcription factor 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    deletion of amino acids 302-437, including the Rb binding domain
  • GenBank ID
  • Entrez Gene
    E2F2 (a.k.a. E2F-2)
  • Tag / Fusion Protein
    • HA-Er (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ctttatccagccctcac
  • 3′ sequencing primer ggaatgtgtgtcagttagggt
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr K. Helin. Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBABE-ER-E2Fs were
gifts from Dr. K. Helin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-ER-E2F2-dRb was a gift from Gerardo Ferbeyre (Addgene plasmid # 62805 ; ; RRID:Addgene_62805)
  • For your References section:

    Regulation of E2Fs and senescence by PML nuclear bodies. Vernier M, Bourdeau V, Gaumont-Leclerc MF, Moiseeva O, Begin V, Saad F, Mes-Masson AM, Ferbeyre G. Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111. 10.1101/gad.1975111 PubMed 21205865