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Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 62858-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 62858-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL4.16
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4758
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Vector typeMammalian Expression, Luciferase
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuciferase
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Alt namefirefly luciferase
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Speciesfirefly
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Insert Size (bp)2290
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Mutationinsertion of chimeric intron at nueotide poclsition 1344 of the firefly luciferase gene; destabilizing sequences (DS) added to the C terminus of the protein (CL1 and PEST) and five consecutive AUUUA elements added to the 3′ UTR (RNA DS ARE) for rapid mRNA turnover
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPromega
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 62858-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 62858-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-LUC2CP/intron/ARE was a gift from Gideon Dreyfuss (Addgene plasmid # 62858 ; http://n2t.net/addgene:62858 ; RRID:Addgene_62858) -
For your References section:
Rapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing. Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G. Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. Epub 2010 Feb 1. 10.1128/MCB.01301-09 PubMed 20123975
Map uploaded by the depositor.
