pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70
(Plasmid
#62944)
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PurposeCreating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0
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Depositing Lab
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Depositing OrganizationNational Institute of Mental Health (NIMH)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 62944-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 62944-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBS-KS-attB1-2-PT-SA-SD-0
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Backbone manufacturerHugo Bellen
- Backbone size w/o insert (bp) 3394
- Total vector size (bp) 5023
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2A-QF2-Hsp70
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Insert Size (bp)1615
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated by the laboratory of Christopher Potter and is based on the QF2 construct (http://www.addgene.org/46115/) published at: http://dx.doi.org/10.1038/nmeth.3250
Other reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.
DNA (Catalog # 62944-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 62944-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70 was a gift from Benjamin White (Addgene plasmid # 62944 ; http://n2t.net/addgene:62944 ; RRID:Addgene_62944) -
For your References section:
Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes. Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.059 PubMed 25732830
Map uploaded by the depositor.
