Skip to main content
Addgene
Open Menu

pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70
(Plasmid #62947)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62947 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 62947-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 62947-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pBS-KS-attB1-2-PT-SA-SD-0
  • Backbone manufacturer
    Hugo Bellen
  • Backbone size w/o insert (bp) 3394
  • Total vector size (bp) 5119

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-LexA::QFAD-Hsp70
  • Insert Size (bp)
    1711

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated by the laboratory of Christopher Potter and is based on the LexA::QFAD construct (http://www.addgene.org/46123/) published at: http://dx.doi.org/10.1038/nmeth.3250

Other reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70 was a gift from Benjamin White (Addgene plasmid # 62947 ; http://n2t.net/addgene:62947 ; RRID:Addgene_62947)

  • For your References section:

    Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes. Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH. Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.059 PubMed 25732830
Related items:
Commonly requested with: