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pRGEB32 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 63142 with HindIII-HF.

Our digest displays the predicted banding pattern using a HindIII-HF single digest: 1 band at ~16kb

*** Note that this gel was at 4 degrees C. for 2 weeks before running again to increase resolution in the 10-20kb range ***

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pRGEB32 with the following enzymes: HindIII-HF and XbaI.

Expected Bands
HindIII-HF: 15.9 kb
HindIII-HF/ XbaI: 9.2 kb & 6.6 kb

We observe the expected bands for this digest.