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Mouse non-targeting control minipool library
(Pooled library #63595)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Pooled Library 63595 Back-ordered
 Shipped as suspended DNA in a microcentrifuge tube 		1 $

Backbone

  • Vector backbone
    lentiGuide-Puro
  • Backbone manufacturer
    Addgene Plasmid #52963
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • Species
    Synthetic
  • Promoter U6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this plasmid does NOT contain Cas9.

Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse non-targeting control minipool library was a gift from Phil Sharp & Feng Zhang (Addgene plasmid # 63595)

  • For your References section:

    Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5. 10.1016/j.cell.2015.02.038 PubMed 25748654
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