pNIC28-Bsa4-iLOVf
(Plasmid #63722)

• Purpose: (Empty Backbone) LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
• Depositing Lab: Brian Smith
• Publication: B Smith Lab iLOF fusion plasmids (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: Opher Gileadi
• Backbone size (bp): 7645
• Modifications to backbone: Inserted translation enhancing element (TEE) and codon optimized iLOV
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • TEE, iLOV, 6xHis, TEV cleavage site (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: T7 polymerase required for expression. Gives very high expression levels, consider reduced (0.1 mM) IPTG for induction and/or reduced induction temperature.
• Copy number: Low Copy

Cloning Information

• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • Complete sequence
• A portion of this plasmid was derived from a plasmid made by: Codon optimized iLOV sequence from Andrew Roe & John Christie

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry