Purpose(Empty Backbone) bait plasmid for an adapted two-hybrid system for C. albicans
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63734||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9231
Vector typeC. albicans bait plasmid
- Promoter Met3
/ Fusion Proteins
- NLS (N terminal on backbone)
- LexA (N terminal on backbone)
- HA (N terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
Note--the ccdB gene contained is inactive as it is missing the start codon so no ccd survival strain is necessary.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC2HB was a gift from Patrick Van Dijck (Addgene plasmid # 63734 ; http://n2t.net/addgene:63734 ; RRID:Addgene_63734)
For your References section:A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Stynen B, Van Dijck P, Tournu H. Nucleic Acids Res. 2010 Oct;38(19):e184. doi: 10.1093/nar/gkq725. Epub 2010 Aug 17. 10.1093/nar/gkq725 PubMed 20719741