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(Plasmid #72606)


Item Catalog # Description Quantity Price (USD)
Plasmid 72606 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pGEM-5Zf(+) [cut with EcoR V and T-tailed to give pGEM-T]
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2964
  • Total vector size (bp) 5300
  • Modifications to backbone
    Part of the pGEM multiple cloning site (between the EcoR V and Apa I sites) was replaced with inserts
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Submitted plasmid was grown in Lucigen E. coli strain "E. cloni 10G"
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP (full)
  • Alt name
    Yeast enhanced green fluorescent protein 3
  • Species
    Aequorea victoria
  • Insert Size (bp)
  • Mutation
    S65G, S72A
  • GenBank ID
    Accession Number U73901

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    OMP decarboxylase
  • Species
    Candida albicans strain 3153A
  • Insert Size (bp)
  • Mutation
    Includes full 819 bp coding sequence of URA3, 435 upstream bases, and 129 downstream bases

Gene/Insert 3

  • Gene/Insert name
    GFP (partial)
  • Species
    Aequorea victoria
  • Insert Size (bp)
  • Mutation
    Includes nucleotides 501-699 of the 717 nucleotide coding sequence of GFP

Gene/Insert 4

  • Gene/Insert name
    Optional Linker
  • Species
    Candida albicans
  • Insert Size (bp)
  • Mutation
    This is a segment of the YWP1 gene that encodes SSASPSGS.

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The yeast enhanced green fluorescent protein 3 ("GFP") portion of this plasmid was obtained from Brendan Cormack (Cormack, Bertram, Egerton, Gow, Falkow & Brown, Microbiology 143: 303-311, 1997); it has the S65G and S72A mutations, is codon-optimized for Candida albicans, and is similar to GenBank Accession Number U73901.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-GFP-URA3-GFP was a gift from Bruce Granger (Addgene plasmid # 72606 ; ; RRID:Addgene_72606)
  • For your References section:

    Accessibility and contribution to glucan masking of natural and genetically tagged versions of yeast wall protein 1 of Candida albicans. Granger BL. PLoS One. 2018 Jan 12;13(1):e0191194. doi: 10.1371/journal.pone.0191194. eCollection 2018. PONE-D-17-36468 [pii] PubMed 29329339