pCVD364
(Plasmid
#63739)
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PurposeV cholerae 395 nanH gene cloned into the BamHI site of pCVD315
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCVD315
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Backbone manufacturerFranklin lab
- Total vector size (bp) 8700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenanH
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SpeciesV. cholerae
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Insert Size (bp)4800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer pBRforEco (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD364 was a gift from James Kaper (Addgene plasmid # 63739 ; http://n2t.net/addgene:63739 ; RRID:Addgene_63739) -
For your References section:
Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli. Vimr ER, Lawrisuk L, Galen J, Kaper JB. J Bacteriol. 1988 Apr;170(4):1495-504. PubMed 2832365
Map uploaded by the depositor.
