pMT-IMD-V5-His
(Plasmid #63748)

• Purpose: Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
• Depositing Lab: Cyril Gueydan
• Publication: pMT-IMD-V5-His (unpublished)

Backbone

• Vector backbone: pMT-V5-His
• Backbone manufacturer: lifetechnologies
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 4340
• Modifications to backbone: none
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: immune deficiency
• Alt name: IMD
• Species: D. melanogaster (fly)
• Mutation: *see comment below
• Entrez Gene: imd (a.k.a. Dmel_CG5576, BG5, CG5576, Dmel\CG5576, IMD, IMd, Imd, anon-WO0172774.166, shadok)
• Promoter: pMT
• Tag / Fusion Protein:
  • V5-6XHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhOI (not destroyed)
• 5′ sequencing primer:  CATCTCAGTGCAACTAAA
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note: Addgene's quality control sequencing finds two amino acid residue substitutions, L47M and H212Y, in the Drosophila IMD insert. These residue changes are not thought to affect plasmid function, and the protein has been shown to activate the Imd pathway as expected.