Purpose(Empty Backbone) yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63781||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerMumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
- Backbone size (bp) 8652
Modifications to backboneAdded 3xHA tag and gateway converted
Vector typeYeast Expression
- Promoter GPD
/ Fusion Protein
- 3xHA (N terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
- Cloning method Gateway Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid a modified form of p415GPD from:
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p415GPD-3xHA-GW was a gift from Susan Baserga (Addgene plasmid # 63781 ; http://n2t.net/addgene:63781 ; RRID:Addgene_63781)
For your References section:A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921