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Luc-Nanog-3'UTRmut
(Plasmid #63894)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63894 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Total vector size (bp) 6563
  • Modifications to backbone
    att L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog homeobox
  • Alt name
    ENK; ecat4; 2410002E02Rik
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1307
  • GenBank ID
    71950 NM_028016.3
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, ecat4)

Cloning Information

Resource Information

Depositor Comments

Mir-34a binding site was mutated to remove the seed region

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Luc-Nanog-3'UTRmut was a gift from Lin He (Addgene plasmid # 63894 ; http://n2t.net/addgene:63894 ; RRID:Addgene_63894)
  • For your References section:

    miR-34 miRNAs provide a barrier for somatic cell reprogramming. Choi YJ, Lin CP, Ho JJ, He X, Okada N, Bu P, Zhong Y, Kim SY, Bennett MJ, Chen C, Ozturk A, Hicks GG, Hannon GJ, He L. Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366. 10.1038/ncb2366 PubMed 22020437