Luc-Sox2-3'UTR
(Plasmid
#63895)
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PurposeLuciferase reporter of mouse wildtype Sox2 3'UTR
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 63895-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 63895-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-Control
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5256
- Total vector size (bp) 6217
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Modifications to backboneatt L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSRY (sex determining region Y)-box 2
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Alt namelcc; ysb; Sox-2
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SpeciesM. musculus (mouse)
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Insert Size (bp)961
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GenBank ID20674
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer GLprimer2
- 3′ sequencing primer RVprimer4
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 63895-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 63895-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Luc-Sox2-3'UTR was a gift from Lin He (Addgene plasmid # 63895 ; http://n2t.net/addgene:63895 ; RRID:Addgene_63895) -
For your References section:
miR-34 miRNAs provide a barrier for somatic cell reprogramming. Choi YJ, Lin CP, Ho JJ, He X, Okada N, Bu P, Zhong Y, Kim SY, Bennett MJ, Chen C, Ozturk A, Hicks GG, Hannon GJ, He L. Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366. 10.1038/ncb2366 PubMed 22020437