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(Plasmid #63897)


Item Catalog # Description Quantity Price (USD)
Plasmid 63897 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 3900
  • Vector type
    Bacterial Expression ; phage display

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    cDNA cloning region
  • Species
  • Insert Size (bp)
  • Promoter lacZ
  • Tag / Fusion Protein
    • Fos (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BssHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AAG ACA GCT ATC GCG ATT GCA G
  • 3′ sequencing primer ACC TAT TGC CTA CGG CAG CCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA library insert was provided by Dr. Gregg Silverman while affiliated with the University of California, San Diego. Currently at New York University School of Medicine

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pJF3H utilizes the backbone of pComb3H and a specially designed cassette for cDNA library display. The cDNA library is expressed as a C-Terminal fusion on Fos. The pIII gene product is expressed as a C-Terminal fusion to Jun. The ompA and pelB leader sequences direct the products to the periplasm where they assembly via Jun/Fos interaction.

Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF3H was a gift from Carlos Barbas (Addgene plasmid # 63897 ; ; RRID:Addgene_63897)
  • For your References section:

    Phage Display: A Laboratory Manual. Barbas CF. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001. Print.