PurposeExpresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64035||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2750
- Total vector size (bp) 6450
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter hSyn
/ Fusion Protein
- ReaChR tagged with mCitrine (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Made by Kim Ritola at Janelia Farm Research Campus, HHMI, for Bryan (Mac) Hooks in Karel Svoboda's lab.
FAS-DO site is for compatibility with FLEX/DIO constructs with traditional loxP sites (Saunders, A., Johnson, C. and Sabatini, B.L. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression. Front. in Neural Circuits 6:47)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hSyn-FAS-DO-ReaChR-mCitrine-WPRE was a gift from Karel Svoboda (Addgene plasmid # 64035 ; http://n2t.net/addgene:64035 ; RRID:Addgene_64035)
Map uploaded by the depositor.