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ROZA-XL
(Plasmid #64194)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64194 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 64194-D50 50 μg of DNA in Tris buffer $495
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DNA 64194-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDNA 3.1+
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5445
  • Total vector size (bp) 1846
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ROZA-XL
  • Species
    Synthetic
  • Insert Size (bp)
    1846
  • Mutation
    ROZA-XL
  • GenBank ID
    KM979358
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct includes a YFP varient "YPET" that I obtained from PS Daugherty, UC Davis, USA (see reference below) and a monomeric variant of CFP that was obtained from RY Tsien UC San Diego, USA (see reference below)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for CFP:
Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916.

Reference for YPET, yellow fluorescent protein:
A.W. Nguyen, P.S. Daugherty, Evolutionary optimization of fluorescent proteins for intracellular FRET, Nat. Biotechnol. 23 (2005) 355e360

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ROZA-XL was a gift from Annemarie Lellouch (Addgene plasmid # 64194 ; http://n2t.net/addgene:64194 ; RRID:Addgene_64194)

  • For your References section:

    ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing Zeta Associated Protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells. Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman JC, Lellouch AC. Biochem Biophys Res Commun. 2015 Feb 28. pii: S0006-291X(15)00357-5. doi: 10.1016/j.bbrc.2015.02.117. 10.1016/j.bbrc.2015.02.117 PubMed 25735979
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