mcherry Gamma 9 ---pcDNA3.1
(Plasmid #64203)

• Purpose: G protein Gamma cone(9) subunit tagged with mcherry on the N terminus
• Depositing Lab: Narasimhan Gautam
• Publication: Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6358
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein Gamma 9
• Alt name: Gng9
• Species: H. sapiens (human)
• Insert Size (bp): 219
• GenBank ID: NM_174328.2
• Entrez Gene: GNGT2 (a.k.a. G-GAMMA-8, G-GAMMA-C, GNG9, GNGT8, HG3I)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mcherry (N terminal on insert)
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mCherry was cloned into the HindIII- KpnI sites of pcDNA3.1 and Gamma cone was cloned into the KpnI--EcoR1 sites of pcDNA3.1. Gamma has a His-tag on its N terminus.