mcherry Gamma 9 ---pcDNA3.1
PurposeG protein Gamma cone(9) subunit tagged with mcherry on the N terminus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64203||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6358
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameG protein Gamma 9
SpeciesH. sapiens (human)
Insert Size (bp)219
- Promoter CMV
/ Fusion Proteins
- mcherry (N terminal on insert)
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
mCherry was cloned into the HindIII- KpnI sites of pcDNA3.1 and Gamma cone was cloned into the KpnI--EcoR1 sites of pcDNA3.1. Gamma has a His-tag on its N terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mcherry Gamma 9 ---pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 64203 ; http://n2t.net/addgene:64203 ; RRID:Addgene_64203)
For your References section:Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Karunarathne WK, Giri L, Kalyanaraman V, Gautam N. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11. 10.1073/pnas.1220697110 PubMed 23479634