8024 GFP_Kras_G12C_2A_puro
(Plasmid #64373)

• Purpose: This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene
• Depositing Lab: Georgios Stathopoulos
• Publication: Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.

Backbone

• Vector backbone: 7432 Bicistronic_GFP_ires_puro
• Backbone manufacturer: Theodora Agalioti
• Backbone size w/o insert (bp): 7432
• Total vector size (bp): 8047
• Modifications to backbone: AgeI, HpaI
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse Kras2A
• Alt name: Kras
• Species: M. musculus (mouse)
• Insert Size (bp): 1356
• Mutation: changed glycine 12 to cysteine
• Entrez Gene: Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: ccctcactccttctctagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was constructed by Theodora Agalioti in G. Stathopoulos Lab in Patras, Greece.