PurposeSUC2 regulated expression of an antisense RNAi construct for GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64404||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4970
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2219
- Promoter SUC2
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PSUC2-GFPantisense-CYC1t-pRS413 was a gift from Claudia Vickers (Addgene plasmid # 64404 ; http://n2t.net/addgene:64404 ; RRID:Addgene_64404)
For your References section:Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae. Williams TC, Espinosa MI, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7. 10.1186/s12934-015-0223-7 [pii] PubMed 25886317