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(Plasmid #64517)

Full plasmid sequence is not available for this item.


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 64517 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5598
  • Total vector size (bp) 6120
  • Modifications to backbone
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Type II hexokinase gene, partial cds and promoter region
  • Alt name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    Isolated from AS-30D rat hepatoma
  • GenBank ID
  • Entrez Gene
  • Promoter Hexokinase II
  • Tag / Fusion Protein
    • firefly luciferase

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GL primer 1
  • 3′ sequencing primer GL primer 2
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For stability the plasmid is maintained in E. coli SURE. For reporter studies it is propagated in E. coli SCS110 or other methylation deficient strain.

Note: Addgene's quality control sequencing has identified a few single nucleotide discrepancies with the depositor's reference sequence. However, the plasmid accurately reflects the construct as it was used in the associated publication, and should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-HKII-E was a gift from Peter Pedersen (Addgene plasmid # 64517 ; ; RRID:Addgene_64517)
  • For your References section:

    Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. Mathupala SP, Rempel A, Pedersen PL. J Biol Chem. 2001 Nov 16;276(46):43407-12. Epub 2001 Sep 13. 10.1074/jbc.M108181200 PubMed 11557773