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(Plasmid #64769)


Item Catalog # Description Quantity Price (USD)
Plasmid 64769 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Promoter TDH3
  • Tag / Fusion Protein
    • fused to the human Estrogen Binding Domain (EBD), which makes the nuclear activity of Cre dependent on the hormone b-estradiol (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (destroyed during cloning)
  • 3′ cloning site PvuII (destroyed during cloning)
  • 5′ sequencing primer GPDpro-F
  • 3′ sequencing primer CYC1
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTW040 was a gift from Fred van Leeuwen (Addgene plasmid # 64769 ; ; RRID:Addgene_64769)
  • For your References section:

    Recombination-induced tag exchange (RITE) cassette series to monitor protein dynamics in Saccharomyces cerevisiae. Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F. G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213. 10.1534/g3.113.006213 PubMed 23708297