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Recombination-induced tag exchange (RITE) cassette series to monitor protein dynamics in Saccharomyces cerevisiae.
Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213. PubMed Article

Plasmids from Article

ID Plasmid Purpose
64751pFvL106Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selection
64753pFvl160Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 epitope tag, Hygro selection
64754pTW073Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection
64757pTW094Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-6xHIS epitope tag, Hygro selection
64758pMT001Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selection
64759pMT004Recombination-Induced Tag Exchange (RITE) Cassette, V5 to an HA-6xHIS epitope tag, Hygro selection
64760pMT005Recombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an HA-6xHIS epitope tag, Hygro selection
64761pMT006Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection
64762pMT010Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection
64763pKV005Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection
64764pKV006Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection
64765pKV014Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 3xT7-mRFP tag, Hygro selection
64769pTW040For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator
64770pSS146For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

Antibodies from Article