pET3a hRegIIIa
(Plasmid #64937)

• Purpose: bacterial expression of processed human RegIIIalpha
• Depositing Lab: Lora Hooper
• Publication: Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8. doi: 10.1074/jbc.M808077200. Epub 2008 Dec 18.

Backbone

• Vector backbone: pET3a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4640
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RegIIIa
• Alt name: HIP/PAP
• Alt name: regenerating islet-derived 3 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 417
• Mutation: processed form. mutations made in 5' end to relax secondary structure.
• Entrez Gene: REG3A (a.k.a. HIP, HIP/PAP, INGAP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BclI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry