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pUC18-mini-Tn7T-Gm-LacZ20
(Plasmid #65027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65027 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pUC18-mini-Tn7T-Gm
  • Backbone manufacturer
    Herbert Schweizer
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • lacZ (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    also Ampicillin resistant
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank Accession # DQ296495

Compared to the Genbank reference sequence DQ296495, Addgene's sequencing results found T920I, D999E and F1000L mutations in lacZ. The effect of these differences is unknown. Note that the amino acid numbering is relative to the lacZ encoded by this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-mini-Tn7T-Gm-LacZ20 was a gift from Herbert Schweizer (Addgene plasmid # 65027 ; http://n2t.net/addgene:65027 ; RRID:Addgene_65027)
  • For your References section:

    mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Choi KH, Schweizer HP. Nat Protoc. 2006;1(1):153-61. 10.1038/nprot.2006.24 PubMed 17406227