pluc-miR148a-148aT
(Plasmid
#65053)
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PurposeReporter plasmid to detect miR148/152 family expression
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Depositing Lab
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Depositing OrganizationInstitut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) (inactive)
Located in Spain -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 65053-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 65053-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL4.13
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4637
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEngineered target sites miR-148a-3p
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)240
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Entrez GeneMIR148A (a.k.a. MIRN148, MIRN148A, hsa-mir-148, mir-148a)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (to EcoRI) (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 65053-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 65053-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pluc-miR148a-148aT was a gift from Cristina Fillat (Addgene plasmid # 65053 ; http://n2t.net/addgene:65053 ; RRID:Addgene_65053) -
For your References section:
miR-148a- and miR-216a-regulated oncolytic adenoviruses targeting pancreatic tumors attenuate tissue damage without perturbation of miRNA activity. Bofill-De Ros X, Gironella M, Fillat C. Mol Ther. 2014 Sep;22(9):1665-77. doi: 10.1038/mt.2014.98. Epub 2014 Jun 4. 10.1038/mt.2014.98 PubMed 24895996
Map uploaded by the depositor.
