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pCMS-eGFP-RTP801-KallR
(Plasmid #65059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65059 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCMS-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDIT4
  • Alt name
    RTP801
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    690
  • Mutation
    The 6 lysines are mutated to arginine - K126R, K152R, K185R, K215R, K216R and K217R
  • GenBank ID
    140942
  • Entrez Gene
    Ddit4 (a.k.a. Rtp801)
  • Promoter CMV-IE
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Apart from the 6 lysines mutated to arginine, there are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMS-eGFP-RTP801-KallR was a gift from Cristina Malagelada (Addgene plasmid # 65059)

  • For your References section:

    Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease. Romani-Aumedes J, Canal M, Martin-Flores N, Sun X, Perez-Fernandez V, Wewering S, Fernandez-Santiago R, Ezquerra M, Pont-Sunyer C, Lafuente A, Alberch J, Luebbert H, Tolosa E, Levy OA, Greene LA, Malagelada C. Cell Death Dis. 2014 Aug 7;5:e1364. doi: 10.1038/cddis.2014.333. 10.1038/cddis.2014.333 PubMed 25101677
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