pEBG-PELP1
(Plasmid #65209)

• Purpose: Full length mammalian expression vector for hPELP1
• Depositing Lab: Ratna Vadlamudi
• Publication: Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75. doi: 10.1158/0008-5472.CAN-10-0628. Epub 2010 Aug 31.

Backbone

• Vector backbone: pEBG
• Backbone manufacturer: Supplied by Addgene
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 9400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PELP1
• Alt name: proline, glutamate and leucine rich protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 3400
• Entrez Gene: PELP1 (a.k.a. MNAR, P160)
• Promoter: EF1a
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EF1aF
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PELP1 cDNA has internal BamHI site