Purposereceiving vector of ORFs for the construction of standard ORF parts in YeastFab work
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65315||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepSMART HcKan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1130
- Cloning method Unknown
- 5′ sequencing primer Lac-promoter
- 3′ sequencing primer ColE1-Rev (Common Sequencing Primers)
the RFP gene was used for screening positive transformants(original plasmids would appear red due to expression of RFP, when RFP was replaced by desired inserts, the colony was white)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HcKan_O was a gift from Junbiao Dai (Addgene plasmid # 65315 ; http://n2t.net/addgene:65315 ; RRID:Addgene_65315)
For your References section:YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J. Nucleic Acids Res. 2015 May 8. pii: gkv464. 10.1093/nar/gkv464 PubMed 25956650