YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
Nucleic Acids Res. 2015 May 8. pii: gkv464. PubMed Article
Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
Nucleic Acids Res. 2015 May 8. pii: gkv464. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65314 | HcKan_P | receiving vector of promoters for the construction of standard promoter parts in YeastFab work |
| 65315 | HcKan_O | receiving vector of ORFs for the construction of standard ORF parts in YeastFab work |
| 65316 | HcKan_T | receiving vector of terminators for the construction of standard terminator parts in YeastFab work |
| 65318 | POT2-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65319 | POT3-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65320 | POT4-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65321 | POT5-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65322 | POT6-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65323 | POT7-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65324 | POT8-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65325 | POT9-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65326 | POT10-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65327 | POT11-RFP | receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly |
| 65328 | pYG026 | reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter. |
| 65329 | pRS414-URR | CEN plasmid as receiving vector for exogenous pathway |
| 65330 | pRS424-URR | high copy number plasmid as receiving vector for exogenous pathway |
| 65331 | pMV-URR1 | standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs |
| 65332 | pMV-URR2 | standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs |
| 65333 | pMV-HIS3 | standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs |
| 65334 | pMV-LEU2 | standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs |
| 65335 | pMV-TRP1 | standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs |
| 65336 | HCKan-P-TDH3 | construction of TDH3 promoter in HCKan-P receiving vector |
| 65337 | HCKan-P-TEF2 | construction of TEF2 promoter in HCKan-P receiving vector |
| 65338 | HCKan-P-CYC1 | construction of CYC1 promoter in HCKan-P receiving vector |
| 65339 | HCKan-T-ADH1 | construction of ADH1 terminator in HCKan-P receiving vector |
| 65340 | HCKan-T-TEF2 | construction of TEF2 terminator in HCKan-P receiving vector |
| 65341 | PMV-ycrtE | systhesis of crtE gene in the beta-carotene pathway |
| 65342 | PMV-ycrtI | systhesis of crtI gene in the beta-carotene pathway |
| 65343 | PMV-ycrtYB | systhesis of crtYB gene in the beta-carotene pathway |
| 65344 | HCKan-O-ycrtE | construction of ycrtE gene in HCKan-O receiving vector |
| 65345 | HCKan-O-ycrtI | construction of ycrtI gene in HCKan-O receiving vector |
| 65346 | HCKan-O-ycrtYB | construction of ycrtYB gene in HCKan-O receiving vector |
| 65347 | pKMV-VioA | systhesis of vioA gene in the violacein pathway |
| 65348 | pKMV-VioB | systhesis of vioB gene in the violacein pathway |
| 65349 | pKMV-VioC | systhesis of vioC gene in the violacein pathway |
| 65350 | pKMV-VioD | systhesis of vioD gene in the violacein pathway |
| 65351 | pKMV-VioE | systhesis of vioE gene in the violacein pathway |
| 65352 | POT2-TDH3p-ycrtE-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65353 | POT2-TEF2p-ycrtE-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65354 | POT2-CYC1p-ycrtE-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65355 | POT4-TDH3p-ycrtI-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65356 | POT4-TEF2p-ycrtI-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65357 | POT4-CYC1p-ycrtI-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65358 | POT5-TDH3p-ycrtYB-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65359 | POT5-TEF2p-ycrtYB-ADH1t | TU for combinatorial construction of ?-carotene pathway |
| 65360 | POT5-CYC1p-ycrtYB-ADH1t | TU for combinatorial construction of ?-carotene pathway |