PurposeCEN plasmid as receiving vector for exogenous pathway
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65329||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAgilent Technologies
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4421
- Cloning method Unknown
- 5′ sequencing primer P1540 BB/S/F-suffix
- 3′ sequencing primer jkRFP-R (Common Sequencing Primers)
pRS414 with URR1-RFP-Leu2-URR2 frament inserted at MCS, used for receiving in vitro ligated pathway by gap-repair method(URR1 and URR2 are homologous arms)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS414-URR was a gift from Junbiao Dai (Addgene plasmid # 65329 ; http://n2t.net/addgene:65329 ; RRID:Addgene_65329)
For your References section:YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J. Nucleic Acids Res. 2015 May 8. pii: gkv464. 10.1093/nar/gkv464 PubMed 25956650