RCAN1
(Plasmid #65413)

• Purpose: Full length RCAN1 expression with CMV promoter. Flag tagged.
• Depositing Lab: Jeffery Molkentin
• Publication: Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 2009 Jan 11.

Backbone

• Vector backbone: p3Xflag-CMV
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5450
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RCAN1
• Alt name: MCIP1
• Alt name: regulator of calcineurin 1
• Species: M. musculus (mouse)
• Insert Size (bp): 756
• Entrez Gene: Rcan1 (a.k.a. Adapt78, CALP1L, CSP1, DSC1, Dscr1, MCIP1, RCN1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag tagged (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry