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pHLSec2-FN-YPet
(Plasmid #65421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65421 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 65421-D50 50 μg of DNA in Tris buffer $495
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DNA 65421-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHLSec2
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 12141
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
  • Alt name
    fibronectin 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
  • Tag / Fusion Protein
    • YPet

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLSec2-FN-YPet was a gift from Harold Erickson (Addgene plasmid # 65421 ; http://n2t.net/addgene:65421 ; RRID:Addgene_65421)

  • For your References section:

    Fibronectin aggregation and assembly: the unfolding of the second fibronectin type III domain. Ohashi T, Erickson HP. J Biol Chem. 2011 Nov 11;286(45):39188-99. doi: 10.1074/jbc.M111.262337. Epub 2011 Sep 23. 10.1074/jbc.M111.262337 PubMed 21949131
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