FUW-ubiquitin-EphB2-SV40-GFP (B2G)
(Plasmid #65442)

• Purpose: Expression of mouse EPHB2 and GFP in mammalian cells
• Depositing Lab: Eduard Batlle
• Publication: Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.

Backbone

• Vector backbone: FUW
• Backbone manufacturer: Addgene Plasmid 14882
• Total vector size (bp): 10365
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPHB2
• Species: M. musculus (mouse)
• Insert Size (bp): 2984
• Mutation: See depositor comments below.
• Entrez Gene: Ephb2 (a.k.a. Cek5, Drt, ETECK, Erk, Hek5, Nuk, Prkm5, Qek5, Sek3, Tyro5)
• Promoter: UBQ
• Tag / Fusion Protein:
  • SV40-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein sequence for EphB2 matches NCBI AAB30284.1. Plasmid should function as described in the associated publication.