FUW-ubiquitin-EphB4-SV40-GFP (B4G)
(Plasmid #65444)

• Purpose: Expression of human EPHB4 and GFP in mammalian cells
• Depositing Lab: Eduard Batlle
• Publication: Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.

Backbone

• Vector backbone: FUW
• Backbone manufacturer: Addgene Plasmid 14882
• Total vector size (bp): 10357
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPHB4
• Alt name: EPH receptor B4
• Species: H. sapiens (human)
• Insert Size (bp): 2963
• Entrez Gene: EPHB4 (a.k.a. CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11)
• Promoter: UBQ
• Tag / Fusion Protein:
  • SV40-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry