mVenus-PA-MKI
(Plasmid
#65457)
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Purposephotoactivatable myosin light-chain kinase inihibition
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 65457-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 65457-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepVenus-C1
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 5230
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKI
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Alt nameMLCKi
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SpeciesSynthetic
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Insert Size (bp)30
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F or GFP-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 65457-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 65457-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mVenus-PA-MKI was a gift from Klaus Hahn (Addgene plasmid # 65457 ; http://n2t.net/addgene:65457 ; RRID:Addgene_65457) -
For your References section:
Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM. ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13. 10.1021/sb5001356 PubMed 24905630