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pHis-zfEpoa
(Plasmid #65611)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65611 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETH2a
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4989
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends strain BL21(DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    erythropoietin
  • Alt name
    Epo
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    486
  • GenBank ID
    NM_001115128.1
  • Entrez Gene
    epoa (a.k.a. e, epo)
  • Promoter T7
  • Tag / Fusion Protein
    • (His)6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-zfEpoa was a gift from Petr Bartunek (Addgene plasmid # 65611 ; http://n2t.net/addgene:65611 ; RRID:Addgene_65611)
  • For your References section:

    Zebrafish kidney stromal cell lines support multilineage hematopoiesis. Stachura DL, Reyes JR, Bartunek P, Paw BH, Zon LI, Traver D. Blood. 2009 Jul 9;114(2):279-89. doi: 10.1182/blood-2009-02-203638. Epub 2009 May 11. 10.1182/blood-2009-02-203638 PubMed 19433857