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pSMART-2,3-BDO1
(Plasmid #65816)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65816 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSMART-HCKan (AF532107.1)
  • Backbone manufacturer
    Lucigen (Middleton, WI)
  • Backbone size w/o insert (bp) 1800
  • Total vector size (bp) 5364
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    budA: α-acetolactate decarboxylase
  • Alt name
    budA
  • Species
    Synthetic; Enterobacter cloacae subsp. dissolvens SDM
  • Insert Size (bp)
    800
  • Promoter waaHp from E. coli as part of operon with budB and budC

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC
  • 3′ sequencing primer CGATGACCGATGTGCTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    budB: acetolactate synthase
  • Alt name
    budB
  • Species
    Synthetic; Enterobacter cloacae subsp. dissolvens SDM
  • Insert Size (bp)
    1700
  • Promoter waaHp from E. coli as part of operon with budA and budC

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCTCCAGTAACCAAATATGCG
  • 3′ sequencing primer TTTGCGCTGCATCCATTAC
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    budC: acetoin reductase
  • Alt name
    budC
  • Species
    Synthetic; Enterobacter cloacae subsp. dissolvens SDM
  • Insert Size (bp)
    750
  • Promoter waaHp from E. coli as part of operon with budA and budB

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTTGCGCTGCATCCATTAC
  • 3′ sequencing primer GGT CAG GTA TGA TTT AA A TGG TCA GT
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMART-2,3-BDO1 was a gift from Michael Lynch (Addgene plasmid # 65816 ; http://n2t.net/addgene:65816 ; RRID:Addgene_65816)