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274_pSKB3-Venus-LUMP
(Plasmid #65976)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65976 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSKB3
  • Backbone size w/o insert (bp) 5245
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LUMP
  • Species
    Photobacterium leignathi
  • Insert Size (bp)
    1353
  • Promoter T7
  • Tags / Fusion Proteins
    • Venus (N terminal on insert)
    • thrombin site (11-residue linker that harbors a thrombin cleavage site (ASLVPRGSRGS) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    274_pSKB3-Venus-LUMP was a gift from Gerard Marriott (Addgene plasmid # 65976 ; http://n2t.net/addgene:65976 ; RRID:Addgene_65976)
  • For your References section:

    Genetically encoded sensors of protein hydrodynamics and molecular proximity. Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G. Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021. 10.1073/pnas.1424021112 PubMed 25931526