Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51960||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1800
/ Fusion Protein
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ateam1.03-nD/nA/pRSETB was a gift from Takeharu Nagai (Addgene plasmid # 51960 ; http://n2t.net/addgene:51960 ; RRID:Addgene_51960)
For your References section:Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators. Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T. ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z. 10.1021/cb900263z PubMed 20047338
Map uploaded by the depositor.