Burden Monitor phi80 version
(Plasmid
#66074)
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PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell
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Depositing Lab
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Depositing OrganizationImperial College London
Located in United Kingdom of Great Britain and Northern Ireland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 66074-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 66074-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAH153
- Backbone size w/o insert (bp) 2323
- Total vector size (bp) 3162
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsPIR+ strain is required for R6K conditional origin of replication
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameBurden Monitor: constitutive BBa_J23100 promoter-strong RBS- superfolder GFP without tag- BBa_1002 terminator
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Insert Size (bp)828
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene NGS identified additional Gentamicin and ori sequences in the backbone as compared to the original depositor sequence. Please see the posted Addgene and Depositor sequences for reference. The impact of this additional sequence is unknown, but the insert that is integrated into the genome is unaffected.
DNA (Catalog # 66074-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 66074-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Burden Monitor phi80 version was a gift from Tom Ellis (Addgene plasmid # 66074 ; http://n2t.net/addgene:66074 ; RRID:Addgene_66074) -
For your References section:
Quantifying cellular capacity identifies gene expression designs with reduced burden. Ceroni F, Algar R, Stan GB, Ellis T. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6. 10.1038/nmeth.3339 PubMed 25849635
Map uploaded by the depositor.
