|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66571||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12448
Vector typeYeast Expression
Growth in Bacteria
SpeciesA. thaliana (mustard weed)
Insert Size (bp)2724
MutationPhyB portion contains aa's 1-908
Entrez GenePHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
- Promoter pADH1
/ Fusion Proteins
- mCherry (C terminal on insert)
- Tom7 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer mCherry F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PhyB-mCherry-Tom7 was a gift from Orion Weiner (Addgene plasmid # 66571 ; http://n2t.net/addgene:66571 ; RRID:Addgene_66571)
For your References section:Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. Jost AP, Weiner OD. ACS Synth Biol. 2015 Jun 2. 10.1021/acssynbio.5b00053 PubMed 26035630