PurposeInntag 6: a small self-structured epitope aimed at innocuous protein tagging
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66870||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II SK+
- Total vector size (bp) 3070
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameInntag 6
Alt namePDB_ID 1WKX
Insert Size (bp)129
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GTTGTG TGGAATTGTGAGCG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-IT6 was a gift from Marti Aldea (Addgene plasmid # 66870 ; http://n2t.net/addgene:66870 ; RRID:Addgene_66870)
For your References section:Inntags: small self-structured epitopes for innocuous protein tagging. Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R, Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M. Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556. 10.1038/nmeth.3556 PubMed 26322837