LOC8R-PAGFP
(Plasmid #66898)

• Purpose: PAGFP targeted to mitochondria using the synthetic LOC8R presequence
• Depositing Lab: Georg Ramm
• Publication: Mitochondrial LOC8R Plasmids (unpublished)

Backbone

• Vector backbone: pPSmOrange-N1
• Backbone size w/o insert (bp): 3956
• Total vector size (bp): 5075
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LOC8R-PAGFP
• Alt name: Mitochondrially targeted photoactivatable green fluorescent protein
• Species: Aequorea victoria
• Insert Size (bp): 1128
• Tag / Fusion Protein:
  • LOC8R (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)