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HaloTag-G protein beta-1
(Plasmid #67016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67016 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 67016-D50 50 μg of DNA in Tris buffer $495
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DNA 67016-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHT2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3988
  • Total vector size (bp) 5977
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag-G protein beta-1
  • Alt name
    transducin beta
  • Alt name
    GNB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1989
  • Entrez Gene
    GNB1 (a.k.a. HG2A, MDS, MRD42)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EBV Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Beta-1 cDNA obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville PA. HaloTag pHT2 was purchased from Promega.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Beta-1 insert can be retrieved from pHT2 using Ngo MIV.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HaloTag-G protein beta-1 was a gift from Catherine Berlot (Addgene plasmid # 67016 ; http://n2t.net/addgene:67016 ; RRID:Addgene_67016)
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