pFBCPDL6
(Plasmid #67285)

• Purpose: CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression
• Depositing Lab: Aziz Sancar
• Publication: Selby et al Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub 2011 Dec 27.

Backbone

• Vector backbone: pFastbac, modified
• Backbone manufacturer: Gibco BRL
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 8000
• Modifications to backbone: yes
• Vector type: bacterial vector, insert not expressed

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CPD photolyase
• Alt name: phr
• Species: D. melanogaster (fly)
• Insert Size (bp): 1665
• Mutation: V275E*
• GenBank ID: NT_033778.4
• Entrez Gene: phr (a.k.a. Dmel_CG11205, C2PHR, CG11205, CG11205-PA, Dm CPD, Dmel\CG11205, Phl, anon-OV1, anonOV1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: Polyhedrin-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct is intermediate in making insect cell virus for expression. *Mutation was found during quality control and is not known to confer any functional change.