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pSuper-PICK1 shRNA
(Plasmid #67522)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67522 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSuper
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 3176
  • Total vector size (bp) 3213
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PICK1
  • gRNA/shRNA sequence
    GCCTCACCATCAAGAAGTACC
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • GenBank ID
    NM_053460.1 NM_008837.3
  • Entrez Gene
    Pick1 (a.k.a. Prk, Prkcabp)
  • Entrez Gene
    Pick1 (a.k.a. Prkcabp)
  • Promoter H1 Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer AATACGACTCACTATAG
  • 3′ sequencing primer CTTTAGTGAGGGTTAAT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper-PICK1 shRNA was a gift from Victor Anggono & Richard Huganir (Addgene plasmid # 67522 ; http://n2t.net/addgene:67522 ; RRID:Addgene_67522)
  • For your References section:

    PICK1 loss of function occludes homeostatic synaptic scaling. Anggono V, Clem RL, Huganir RL. J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011. 10.1523/JNEUROSCI.5633-10.2011 PubMed 21307255