Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pER-rxRFP1.4
(Plasmid #67842)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67842 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rxRFP1.4
  • Species
    Synthetic
  • Insert Size (bp)
    996
  • Promoter CMV
  • Tags / Fusion Proteins
    • ER localization (N terminal on insert)
    • His Tag (N terminal on insert)
    • ER retention (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pER-rxRFP1.4 was a gift from Huiwang Ai (Addgene plasmid # 67842 ; http://n2t.net/addgene:67842 ; RRID:Addgene_67842)
  • For your References section:

    Development of redox-sensitive red fluorescent proteins for imaging redox dynamics in cellular compartments. Fan Y, Ai HW. Anal Bioanal Chem. 2016 Jan 12. 10.1007/s00216-015-9280-3 [pii] PubMed 26758595